Effects of Moringa oleifera on Total protein and lactate Dehydrogenase levels in testiculotoxic rats treated with cadmium chloride
Keywords:
Testicular toxicity, cadmium, Moringa oleifera, total protein, lactate dehydrogenaseAbstract
Background: The present study aimed at investigating the influence of Moringa oleifera (MO) on the levels of total protein and lactate dehydrogenase in cadmiuminduced testicular toxicity.
Methods: Twenty-one adult male wistar rats were randomly divided into 3 groups, A, B and C (n=7) which were treated every day regularly for three weeks. Group A (Control) rats received 2ml per day 0.9% w/v Normal saline orally. Group B rats were treated with cadmium chloride 2.5 mg/kg bwt subcutaneously while group C rats were pre-treated orally with extract of MO 500 mg/kg bwt before treating with cadmium chloride 2.5 mg/kg bwt subcutaneously. Blood samples were collected prior sacrifice and sera used for estimations of total protein (TP) concentrations and lactate dehydrogenase (LDH) activities. Testes were harvested and homogenates used for estimation of TP concentrations and LDH activities. Weights of the testes were also recorded.
Results: The results showed significant (p<0.05) decrease in the weights of testes of rats treated with cadmium chloride when compared to control and pre-treated rats. There were significant (p<0.05) reductions in the levels of testicular TP and LDH activities in rats treated with cadmium chloride but sera TP concentrations and LDH activities were significantly (p<0.05) increased. However, pre-treatment with extract of MO restored TP concentrations and LDH activities to near normal values.
Conclusion: We concluded that leaves of Moringa oleifera possess potentials to restore deranged levels of LDH and TP observed in heavy metal-induced testicular toxicities.
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