Invitro antioxidant capacity, phytochemical screening and Gas chromatography GC-MS analysis of Ethanol root extract of Sphenocentrum jollyanum (Pierre)
Keywords:
Sphenocentrum jollyanum, free radicals, phytochemicals, phenols, Octadecadienoic acidAbstract
Background: In-vivo antioxidants potentials of Sphenocentrum jollyanum had been reported in disease conditions. However, there is a paucity of information on its invitro capacity compared to standard antioxidants. The objective of the study is to screen ethanol root extract of Sphenocentrum jollyanum (SJE) for its invitro antioxidants capacity and phytochemical components.
Methodology: Antioxidant capacity and phytochemical content were determined using ABTS, DPPH and GC-MS techniques.
Results: Free radicals were scavenged by SJE in all the In vitro methods used in a concentration dependent manner. Howbeit, the free radical scavenging ability and reducing power potentials of SJE were significantly lower (p<0.05) when compared to standard antioxidants such as Trolox and Vit.E The qualitative screening shows the presence of alkaloids, phenols, phlobatanins, saponins, tannins, amino acid and reducing sugar while GC-MS revealed thirty-four plant bioactive principles among which are members of Octadecadienoic acid family including 12-Octadecadienoic acid, ethyl ester also known as Linoleic acid.
Conclusion: Sphenocentrum jollyanum ethanol root extract possesses antioxidant capacity and important phytochemicals that can be useful in the design, synthesis, and advancement of new medications.
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