Alcohol-Diazepam Combination: Implication on the Histoarchitectural Profile of the Hippocampus in Male Rats.
Keywords:
Addiction, abuse, use, dependence, drugAbstract
Objective: Damage to hippocampal neurons has not been described as a constant finding in studies addressing the effects of alcohol and diazepam in laboratory based studies, the present study aimed to elucidating the changes in the histoarchitectural changes of the hippocampus following separate and combined diazepam and alcohol administration in male Wistar rats.
Methodology: Twenty male Wistar rats were randomly divided into four groups (n=5): control (A), 3 mg/kg diazepam (B), 30% v/v ethanol (C), and 30% v/v ethanol plus 3 mg/kg diazepam (D). All administration was done orally for 30 days.
Major findings: There were no distortions in the hippocampal profile of the rats in the control group. The histoarchitectural profile of the hippocampus of the rats in group B showed less significant damage. The histoarchitectural profile of the hippocampus of the rats in group C was disrupted with evidence of enlarged cell bodies and distorted membrane. The hippocampal profile of the rats in group D were with marked neuronal necrosis and variable neuronal loss within the hippocampal subfield.
Conclusion: It was observed that co-administration of ethanol and diazepam conferred neurodegenerative effects on the neuronal profile of the hippocampus in rats.
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