Prevalence of Gram-negative bacteria and their antibioticresistance pattern at tertiary care hospital Amreli Gujarat India

K Sanjeev; V Krina

doi:10.4314/rejhs.v11i1.2

Authors

K Sanjeev https://orcid.org/0000-0002-5644-4546
V Krina

DOI:

https://doi.org/10.4314/rejhs.v11i1.2

Keywords:

Gram-negative bacteria, clinical samples, antibiotic resistant pattern, multi-drug resistant

Abstract

Background: Infections with multi-drug resistant (MDR) bacteria are serious threats to healthcare services many low-income countries associated with overuse and misuse of antibiotics.

Material and Methods: A prospective cross-sectional study was done between January, 2021 to December, 2021 at Shantabaa Medical College and General Hospital, Amreli, Gujarat, India. Clinical specimens such as swab from wound, urine, sputum, blood, body fluids and throat swab were sampled following standard operating procedures.

Results: A total of 738 patient specimens were collected and processed from different clinical samples such as urine 540 (73%), wound swab 48 (6.5%), sputum 96 (13%), blood 06 (1%), throat swab 12(1.5%)and other body fluids 36 (5%). Overall, 142 (19.2%) of the specimen collected were culture positive. From the 142 positive patients, the proportion of bacterial isolates was higher in females 95(67%), than males 47 (33%). Out of 142 culture positive isolates, Eshcerchia coli (E.coli) 56 (39%), followed by Pseudomonas aeruginosa 35 (25%), Klebsiellae 24 (17%), Enterobacter 12 (8%), Citrobacter 8(6%), Acinetobacter 5(4%) and Serratia 2(1%).

Conclusion: E.coli followed by Pseudomonas aeruginosa, Klebsiellae, and Enterobacter were the most commonly isolated gram-negative bacteria. These bacteria were resistant to the routinely used antibiotics. Therefore, clinicians should practice rational choice of antibiotics and treatment should be guided by antimicrobial susceptibility testing.

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